A Homogeneous in Vitro Functional Assay for Estrogen Receptors: Coactivator Recruitment
نویسندگان
چکیده
منابع مشابه
Allosteric regulation of estrogen receptor structure, function, and coactivator recruitment by different estrogen response elements.
Hormone-activated ERs (ERalpha and ERbeta) bind with high affinity to specific DNA sequences, estrogen response elements (EREs), located within the regulatory regions of target genes. Once considered to function solely as receptor tethers, there is an increasing amount of recent evidence to suggest that the sequence of the ERE can influence receptor activity. In this study, we have performed a ...
متن کاملImmunocytochemical Assay for Estrogen Receptors Applied to Human Prostatic Tumors1
An immunocytochemical assay using a monoclonal antibody to estro gen receptor was applied to frozen sections of 43 prostatic tumors. In addition, in 16 tumors the results of the immunocytochemical assay were compared with those obtained using an enzyme immunoassay for estrogen receptor, a tritiated ligand binding assay, and a histochemical procedure using fluorescein-labeled estradici conjugate...
متن کاملImmunocytochemical assay for estrogen receptors applied to human prostatic tumors.
An immunocytochemical assay using a monoclonal antibody to estrogen receptor was applied to frozen sections of 43 prostatic tumors. In addition, in 16 tumors the results of the immunocytochemical assay were compared with those obtained using an enzyme immunoassay for estrogen receptor, a tritiated ligand binding assay, and a histochemical procedure using fluorescein-labeled estradiol conjugates...
متن کاملElectrophoretic assay of specific estrogen receptors: a contribution to methodology.
Experimental evidence is presented that supports the use of the cold agar-gel electrophoretic method for the clinical quantitation of specific estrogen-binding protein present in some human mammary carcinomas. It is necessary to dilute tumor extracts to avoid interference by serum-borne, non-relevant hormone-binding proteins such as albumin, which migrates to the same anodal region as does the ...
متن کاملProliferation Receptors and Estrogen-Dependent Breast Cancer Cell Ada3 Requirement for HAT Recruitment to Estrogen
We have previously shown that evolutionarily conserved alteration/deficiency in activation (Ada) protein associates with and promotes estrogen receptor (ER)–mediated target gene expression. Here, we examined the role of endogenous Ada3 to recruit histone acetyl transferases (HAT) to an ERresponsive promoter and its role in estrogen-dependent cell proliferation and malignant phenotype. Using a c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Endocrinology
سال: 2003
ISSN: 0888-8809,1944-9917
DOI: 10.1210/me.2002-0331